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Oocyte Perfusion Chamber OPC
Expression of recombinant receptors in oocytes have been one of the favorite
choices for researchers to assess the pharmacology and physiology of receptors.
Since the oocytes are 1 - 1.5 mm in diameter, however, the fast solution
exchange around the oocyte can be a problem. After several years of
electrophysiological research, we have designed a Xenopus oocyte
perfusion chamber for use in automated experiments. One of the major characteristic of the chamber
presented is a small working volume (~10 μl), which allows faster solution
exchanges. Combined with perfusion systems, this
chamber lets you to obtained dose-response data fast and easily. It can be used with Oocyte Voltage Clamp amplifiers, which employ
one or two
electrodes bath clamp. Chambers with replaceable 24x24mm glass bottom are
designed to perform imaging using inverted microscopes. The chambers
can be used with perfusion manifolds. The
incorporated suction tubing requires connection to an outflow unit
to remove solution during perfusion.
Improved design makes using the chamber easier: the replaceable chamber is fixed inside a
microscope stage adapter using flat springs. Improved profile of the suction
tubing provides smooth solution flow. Comes with Allen wrench. Click on image to enlarge.
Sample publications:
6,
7,
4,
5,
1,
2,
3
Specifications: |
Dimensions: | 2mm
x 3mm |
Media Exchange: | fits
both TPM and zero-dead volume manifold ZMO for faster solution application |
Working volume: | approx.
10 microl |
Click on catalog numbers below to purchase online.
Required accessories: zero-dead volume manifold, or
PTFE perfusion manifold.
Optional accessories: perfusion system,
flow control.
Download PDF manual.
Download PDF catalog.
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Bioscience Tools
ph: 877-853-9755, fax: 866-533-7490
ph/fax: 1-619-793-4467 (from outside USA)
fax: 866-533-7490
email: info@biosciencetools.com
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PRICES AND OPTIONS |
OPC3 |
$295 |
Oocyte Perfusion Chamber
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OPC3-M |
$395 |
Oocyte Perfusion Chamber on Magnetic Stands.
This modification of OPC3 chamber allows to position oocytes directly
on a bench using three miniature magnetic stands. The microscope free
configuration isolates the oocytes from vibrations, which could be
transmitted through microscope. Inexpensive dissecting microscopes
on a swing arm could be employed in the set-up.
If you do not have steel magnetic surface, choose slider-plate option,
which allows to bolt three adjustable plates to any surface with
wholes, or consider steel plate bellow. PTFE
manifold is optional.
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OPC3-P |
$395 |
Oocyte Perfusion Chamber on Slider-Plates.
Includes M6 and 1/4-20 mounting screws.
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OPC4-108 |
$295 |
Oocyte Perfusion Chamber with Glass Bottom, Nikon 108mm adapter. Replaceable 24x24mm glass coverslip allows you to perform
fluorescence imaging using inverted microscopes |
OPC4-110 |
$295 |
Oocyte Perfusion Chamber with Glass Bottom, Olympus 110mm adapter. Replaceable 24x24mm glass coverslip allows you to perform
fluorescence imaging using inverted microscopes |
CS-No2-24x24 |
$25 |
No. 2 Glass Cover Slip, 24x24 mm , box of 100. Optical quality no. 2 glass for OPC4 oocyte chamber. 24 x 24 mm
square. |
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