Coverslips > Glass Bottom Dishes
Imaging > Glass Bottom Dishes > Glass Bottom Dishes
Glass Bottom Dishes vs. Petri Dishes

Glass bottom dishes are used with short working distance, high N.A. objective in fluorescence, confocal and image analysis experiments. Polarization free, does not distort Differential Interference Contrast. The outside shape and glass flat bottom provides a parallel (optical) fit in the microscope stages and good thermal conductivity in heated platforms (below.) The inside well allows you to use only small volumes of media, if necessary: See schematics. Click on image to enlarge. Click for glass coating procedures.

The heating elements with reduced optical window in the middle (down to 15 or 11mm) will provide better temperature stability for your sample, and are perfect for use with glass bottom dishes and long-working-distance objectives. If immersion objectives are used, however, this small window will limit access of the large immersion objective to the whole bottom surface. In this case a heating element with 35mm clearance/window TC-E35 should be used. Temperature stability still can be provided by using an objective heater.

In contrasts to glass bottom dishes, which provide good thermal contact because of flat glass bottom, standard plastic Petri dishes very often have a protruding rim alone the bottom edges of the dish. This rim elevates the dish above heating surface. Metal shims/washes can be used to eliminate this air gap, however. This will reduce access for the immersion optics to the bottom of the dish, so this can be used with long-distance objectives only. If using immersion optics and large objectives, 35mm heating element TC-E35 should be used, combined with an objective heater. Please note, that some glass bottom dishes, Mattek dishes for example, are fabricated from standard Corning (or Falcon dishes).

Since different brands of dishes have different outside diameter, sometimes larger than 38mm, we make reducing inserts to provide better fit and thermal contact to the inside cutout of the heating elements, which is 38mm. We also make a 50mm insert to center the dishes inside incubators and larger heating elements, which have 50mm inside cutout.

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Bioscience Tools
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PRICES AND OPTIONS

TC-PA-C

$30

Reducing adapter-ring, for Corning type dishes.

TC-PA-N

$30

Reducing adapter-ring, for Nunc type dishes.

TC-PA-W

$30

Petri Dish Adapter. Petri dish adapter -reducing ring- to use the controlled environment setup with different brand petri dishes. The adapters fit inside the heater and fits around the 35mm WillCo dish to eliminate air gaps and to prevent dish movement.

TC-PA-F

$30

Reducing adapter-ring, for FluoroDishes.

TC-PA50

$30

50mm reducing adapter for 35mm dishes, to use with TC-MIS, TC-MI incubators and TC-E50 heating elements.

PDI

$95

Low Profile Chamber-Insert for Petri dish

PCP2-W

$30

Glass Bottom WillCo Dish Insert. Modification of PDI insert for standard petri dishes. Facilitates perfusion inside the dish. Separate inflow and outflow compartments prevent bubbles from entering the working compartment and provide smooth perfusion. Can be used with perfusion systems. See sample recordings. Choose dish size when ordering (35 mm dish with 12 mm.)

LPPCP1-W

$165

Glass Bottom WillCo Dish Perfusion Setup. In addition to the PCP2 insert, includes a set of magnetic holders and stainless suction tubing. Choose microscope adapter when ordering.

W-HLD

$29

Petri Dish Magnetic Clamps. These two clamps could be positioned anywhere on the stainless steel microscope adapter of the setup above and fix the dish firmly in place. Can be used to prevent dish elevation while working with oil immersion objectives.

MA

$75

Magnetic microscope adapter, specify microscope model when ordering.

TC-PD-35x15w

$155

Heater for 35mm glass bottom dishes. Ready to use temperature controlled system for WillCo dishes. In order to eliminate temperature gradient, the dish is heated from the bottom. Optical clearance is 15mm diameter (the heater with 11mm clearance for 12mm wells is also available). If larger clearance is required, a 35mm heater for glass coverslips should be used. The dish can remain covered during observation. Built-in sensor for temperature stability and overheating protection. An external temperature probe of temperature controllers can be positioned inside the dish if needed using included miniature holder. Requires a microscope adapter (specify microscope model when ordering). Requires a temperature controller.

TC-PD-50x40

$335

Heater for 50mm dishes with 40mm optical clearance. This system is similar to the above, but for 50mm glass bottom dishes. Optical clearance is 40mm. For smaller clearance choose the system below. Specify microscope adapter when ordering. Can be used with perfusion inserts (miniature holders might be required to position solution tubing). Requires a temperature controller.

TC-PD-50x30

$335

Heater for 50mm dishes with 30mm optical clearance. Optical clearance in this system is 28.5mm. Specify microscope adapter when ordering. Requires a temperature controller.

TC-CIC-15

$495

Closed Controlled Environment for Petri Dishes. Uses polarization-free glass coverslips to form optical quality surfaces to control media, atmosphere (pH), and to prevent condensation. Perfect for fluorescence imaging. Easy to assemble. Has inside reservoir for water to prevent media evaporation during long time imaging. Incorporates luer connector for gas mixture input. Includes a set of adjustable tubing holders to position perfusion tubing for continuous media exchange. The included petri dish insert (fits glass bottom dishes) has separate compartments for solution inflow and outflow to provide smooth perfusion and to prevent bubbles from entering the working area. Consider adding a miniature perfusion and controlled flow system. Included Teflon inside tubing preheats solution, and fits perfusion systems. Can be upgraded with an objective heater for immersion optics. Can be used with standard 35mm petri dishes (optional petri dish adapters and perfusion insert might be required). The system can be also used with coverslip holders (replaceable coverslips) for high resolution imaging. Requires a microscope adapter. Specify microscope model when ordering. Requires a temperature controller.