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Coverslips > Perfusion Chambers > Low Profile Glass Bottom Chamber
Imaging > Coverslip Chambers > Low Profile Glass Bottom Chamber
Media Exchange > Perfusion Chambers > Low Profile Glass Bottom Chamber


Low Profile Perfusion Chamber for Glass coverslips

The most flexible and easy to use perfusion chamber-assembly for upright and inverted microscopes. To accommodate the wide range of possible applications, this system consists of three movable and replaceable elements: bath chamber, magnetic adjustable tubing/electrode holders and a stainless microscope stage adapter-insert. It can be used with upright or inverted microscopes, fluorescent or water immersion objectives, tissue slices or single cells, and an be used as stand alone or can be easily adapted to perfusion systems.

The chamber is made out of polycarbonate. It is only 4 mm dip. This low profile fits under objectives of upright microscopes. The I.D. of working area is 21 mm, wide enough to work with tissue slices. In fact, this design is popular among researchers, who work with brain slices. Water immersion objectives can be easily positioned inside the wide opening. A small volume version of the chamber is also available. The bottom of the chamber is a replaceable no.1 glass coverslip, which lets you to use the chamber with inverted/fluorescent microscopes as well. The coverslip is attached to the chamber by using provided adhesive layers. Coverslip is flashed on the bottom of the chamber. Easy to clean after use. Note: Can be used with any thickness 25mm coverslips.

The chamber fits inside stainless microscope stage adapter, specify microscope model when ordering. The chamber can be rotated inside the stage to provide required orientation of the sample. Custom stages for non-standard microscopes are available upon request.

Three magnetic tubing/electrode holders extend this flexibility even further. They can be positioned anywhere around the chamber, so that tubing is not in the way of objectives or recording electrodes. Furthermore, the holders can adjust the angle and, as a result, tubing can approach the sample from any direction. The holders fit to perfusion manifolds. They also can be used to position reference electrodes or temperature probes, or even tubing to deliver gases, for example oxygen, over the sample. Included stainless suction tubing provides smooth perfusion. If you are planning to use this system during perfusion experiments, all you need is a outflow unit for suction to remove solution during perfusion. Can be used for imaging and recording. Click on image to enlarge. 3-D Model. Sample publications 1, 2.

Specifications:

Outside diameter:

35mm

Height:

4mm

Working volume:

21mm diameter, approx. 350 microl



Click on catalog numbers below to purchase online.

Required accessories: microscope adapter, miniature tubing holders, 25mm coverslips, adhesive layers.

Optional accessories: perfusion system, flow control, temperature control, objective heater.

Download PDF manual.

Download PDF catalog.

Bioscience Tools
ph: 1-877-853-9755
ph: 1-858-537-1062 (from outside USA)
fax: 1-619-582-0919
email: info@biosciencetools.com


PRICES AND OPTIONS

PCCS1

$60

Low profile Chamber for Glass coverslips.

CS-No1-25

$15

Glass Cover Slip, box of 100. Optical quality glass cover slip for perfusion bath chambers. Box of 100. Made in Germany. No. 1 thickness.

UTIC-21

$80

Adhesive layers, pack of 100.

MA-MTH

$135

Miniature Magnetic Holders Set, x3. Includes stainless suction tubing.

MA

$15

Magnetic microscope adapter. Specify microscope model when ordering.