Temperature controllers
 
Media Exchange
Solution Applications
Petri Dish Perfusion
 
CO2 control
 
Objective Heaters
 
Ultra-Thin Chambers
 

Miniature Incubators > Incubator for Slides and Petri Dishes
Imaging > Miniature Incubators > Incubator for Slides and Petri Dishes

Miniature Incubator for standard microscopes to use with slides and petri dishes, TC-MIS

Can be used with petri dishes, chambered coverglasses, coverslip holders and glass bottom dishes, both 35 and 50mm. Incorporates luer-lock port for gas mixture (to control CO2 or hypoxia), inside reservoir for water to control humidity, and heated cover, to prevent condensation. Built-in multiple ports for tubing and accessories (probes and sensors). Requires a temperature controller. Incorporates STAGE sensor for stable temperature control, and INTERNAL sensor to monitor inside temperature. Fits any microscope (specify microscope model when ordering, ships installed on the microscope adapter; some Zeiss microscopes need a recessed down insert - type K - and use a different incubator with 160x110mm foot print).

Can be upgraded with an objective heater for immersion optics. For use with long-distance objectives, select TC-MIS-25x70-HB incubator with closed 1mm heated glass bottom, which allows you to use both slides and petri dishes. Can be used for long-term imaging. Can be used with multi-channel solution switch and perfusion systems. An optional set of adjustable tubing holders MH-MIS allows you to position inflow and outflow tubing for continuous media exchange. See cell chemotaxis tracks obtained during 8 hours incubation. Click on images to enlarge.

Low-Profile configurations ( /L) are only 25mm high and can be used with microscope condensors that require low vertical positioning to obtain bright-field images with Phase-contrast and DIC optics. Incorporates two ports for CO2 input and to replenish water to control humidity inside. Although this configuration provides two ports that can be used for tubing, it is not recommended for perfusion applications due to reduced vertical clearance inside.

Incubator with 22x46mm bottom window, TC-MIS-20x46/L. Can be used with 50mm dishes and chambers, slides/coverglasses, and, in combination with 50mm reducing insert TC-PA50 (-C, -N, -F, -W), with 35 dishes and coverslip holders CSC/UTIC.


Incubator with 35mm bottom window for use with petri dishes and coverslip holder CSC and UTIC, TC-MIS-35/L. Has the whole bottom open for easy access with immersion optics.

Incubator with 15mm bottom window for use with petri dishes, TC-MIS-15/L. Provides heat from the bottom. Might require reducing insert TC-PA-C/F/N/W for different brands of petri dishes.

Incubator with 11mm bottom window for use with petri dishes, TC-MIS-11/L. Provides heat from the bottom. Might require reducing insert TC-PA-C/F/N/W for different brands of petri dishes.

Incubator with 45mm bottom window for use with 50mm petri dishes and chambers, TC-MIS-45/L. If used with 50mm reducing insert TC-PA50, can be also used with 35nn dishes and coverslip holders CSC and UTIC.

Incubator with 30mm bottom window for use with 50mm petri dishes and chambers, TC-MIS-30/L. Provides heat from the bottom. If used with 50mm reducing insert TC-PA50, can be also used with 35nn dishes and coverslip holders CSC and UTIC.

Incubator with 25x70mm bottom window and 1mm heated closed bottom for use with slides, and petri dishes, TC-MIS-25x70-HB. For use with long-distance objectives only. Has the whole bottom closed with 1mm glass to provide uniform heating. If used with 50mm reducing insert TC-PA50, can be also used with 35nn dishes and coverslip holders CSC and UTIC.

Specifications:

Stability:

0.01°C

Outside dimensions:

110 x 110 mm; 34mm from sample plane to the top cover (25mm clearance for low-profile configuration)

Optical window (on the bottom):

20x46mm, 25x70mm, 40, 35, 30, 15 and 11mm diameter

Use with:

50mm and 35mm glass bottom dishes, and 1x3in. coverglasses/slides, coverslip holders CSC and UTIC

Condensation free lid:

Uses a built-in temperature sensor to connect independently to the second channel of temperature controllers; 70x70mm optical window

CO2 port:

Luer-lock connector to a source of gas mixture.

Water reservoir:

Controls humidity. Can be replenished through outside ports.

Media exchange and perfusion:

Multiple sealed inflow/outflow ports, a set of optional adjustable tubing holders to position inflow and outflow tubing inside sample chambers.



Perfusion cover low-profile, with perfusion ports and tubing, FLOW-LOW

For use inside TC-MIS incubators. Includes x2 sets of tubing to fit into reduces inflow and outflow ports, which extend to the bottom of a petri dish. Tubing has a luer-lock fitting attached, and can be put through side ports of the incubator. This cover, if placed on a standard Petri dish, forms a flow cell. The high optical quality glass window on top is 12mm diameter. Can be used in combination with PDI insert to provide laminar flow. Can be used to replenish media around your samples during time-lapse imaging. Can be sterilized with ethanol solution or autoclaved (100°C max).

Click on catalog numbers below to purchase online.

Required accessories: temperature controller, microscope adapter, CO2 controller.

Optional accessories: objective heater, perfusion system and accessories, flow control.

Download PDF manual.

Download PDF catalog.

Bioscience Tools
ph: 1-877-853-9755
ph: 1-619-582-1597 (from outside USA)
fax: 1-619-582-0919
email: info@biosciencetools.com


PRICES AND OPTIONS

TC-MIS-20x46/L

$315

Miniature Incubator for Glass Coverglass, Slides and dishes, 20x46mm bottom window

TC-MIS-45/L

$315

Miniature Incubator for 50mm glass bottom dishes, 45mm bottom window

TC-MIS-30/L

$365

Miniature Incubator for 50mm glass bottom dishes, 30mm bottom window

TC-MIS-35/L

$345

Miniature Incubator for 35mm petri dishes and coverslip holders CSC and UTIC, 35mm bottom window

TC-MIS-15/L

$395

Miniature Incubator for 35mm petri and glass bottom dishes, 15mm bottom window

TC-MIS-11/L

$395

Miniature Incubator for 35mm petri and glass bottom dishes, 11mm bottom window

TC-MIS-25x70-HB/L

$325

Incubator with 25x70mm bottom window and 1mm heated closed bottom for use with slides, and petri dishes

IMA-74

$95

Microscope Adapter for Miniature Incubators.

TC-PA50

$30

50mm reducing adapter for 35mm dishes, to use with TC-MIS-20x46, TC-MIS40 and TC-MIS-30 models.

TC-PA-C

$30

Petri Dish Adapter -reducing rings- for Corning and Mattek dishes

TC-PA-N

$30

Petri Dish Adapter -reducing rings- for Nunc dishes

TC-PA-F

$30

Reducing adapter  -reducing rings-, for FluoroDishes.

TC-PA-W

$30

Petri Dish Adapter -reducing rings- for Willco dishes

TC-MI-TP

$95

Adapter cable to connect the incubator lid in parallel with the base to the same channel of a temperature controller, includes cable-extension to monitor inside temperature

MH-MIS

$95

Set of miniature adjustable tubing and sensor holders, x3, includes 4-40 threaded posts

FLOW-LOW

$115

Perfusion cover low-profile, with perfusion ports and tubing

PDI

$95

Low Profile Chamber-Insert for Petri dish