Temperature controller
CO2/O2 control
Objective Heaters
Media Exchange
Solution Applications
Petri Dish Perfusion
Coverslip Chambers

Miniature Microscope Incubators > CO2/O2 Controllers

CO2 and hypoxia controller - CO2-O2-MI

Designed for live cell imaging and time-lapsed microscopy, for use with miniature incubators and custom enclosures. Connects to a cylinders with compressed CO2 gas and balance gas - Nitrogen usually. The output connects directly to a gas port of the incubator or a humidifier. Simple to use through the touch screen. The controller makes 0-20% CO2 and 0-20% O2 mixture to supply inside the incubator. CO2 control at 5% level keeps pH of media constant. The controller ships adjusted for 5% CO2 level requirement. Can be adjusted to fill incubators of different volumes by controlling output flow rate. The instrument not only controls gas content inside the incubator, but also brings the gas consumption to the minimum. For hyperoxia applications, consider another model. Sample publications.



max 100PSI


750 sccm max


INPUT for 5/32in. (4mm) O.D. tubing (included);
OUTPUT for 1/8in O.D. tubing (included);
can be replaced with any 10-32 threaded connectors;
includes luer fitting for different size tubing;
includes easy-disconnect coupling for 1/4in. O.D. tubing


digital display of CO2, O2 and flow levels

Digital Controls:

CO2 level 0-20%

O2 level 0-20%

FLOW up to 750 sccm

INPUT PRESSURE manual regulators, 

CLOSE switches for CO2 and N2, 


Digital display


12 x 6 x 8in.


100-240VAC 75W

Heated humidifier CO2-500ml
A heated platform with 1L reservoir to provide gas saturation with water vapors by passing the gas mixture through heated water. When passed through heated water, CO2 mixture becomes humid. Can be also used as an indicator of gas flow - bubbles will indicated gas flowing inside the incubator. Connects inline between the controller and the incubator.

Heated Water Trap TC-HUM
This heated 5in. long plastic cylinder able to trap water that might come out from humidifiers due to condensation. The temperature control inside the cylinder continuously warms the gas flowing from humidifiers and eliminates temperature fluctuations inside the incubator. The cylinder incorporates an easy disconnect cap to remove water. Small size allows to position the trap next to the incubator on the microscope stage. Includes two luer barbed connectors (male and female) to connect tubing. Includes a 1-channel temperature controller and 10in long flexible heater wrapped around the cylinder. Connects inline between the humidifier and the incubator.

Required accessories: sources of CO2, N2 gases, miniature incubator.

Optional accessories: a humidifier CO2-500ML, Heated water trap TC-HUM.

Download PDF manual.

Download PDF catalog.

Click on catalog numbers below to purchase online.

Bioscience Tools
ph: 877-853-9755, fax: 866-533-7490




CO2/O2 Controller for Miniature Incubators



Heated Humidifier



Heated Water trap with temperature controller

Sample publications:
8 High resolution spatial investigation of intracellular oxygen in muscle cells. bioRxiv, July 19, 2023;
7 Hyperglycemia exacerbates ischemic stroke not through increased generation of hydrogen peroxide. Free Radical Biology and Medicine, Volume 208, 1 November 2023, Pages 153-164;
6 Computational Modeling and Imaging of the Intracellular Oxygen Gradient. Int. J. Mol. Sci. 2022, 23(20), 12597;
5 Mitochondrial respiration reduces exposure of the nucleus to oxygen. JBC, February 14, 2023;
4 Intracellular imaging of metmyoglobin and oxygen using new dual purpose probe EYFP-Myoglobin-mCherry. Journal of Biophotonics, Volume15, Issue3, March 2022;
1 Intracellular oxygen mapping using a myoglobin-mCherry probe with fluorescence lifetime imaging. J. Biomed. Opt. 23(10), 107001 (2018);
2 Single cell-based fluorescence lifetime imaging of intracellular oxygenation and metabolism; Redox Biol. 2020 Jul; 34: 101549;
3 Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry. Methods in Molecular Biology, 01 Jan 2021, 2304:315-337;
4 In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model. j.redox.2021.102178;