BIOSCIENCE TOOLS, 4527 52nd Street, San Diego, CA 92115
Ph: 877-853-9755,
Ph: 1-858-537-1062 (from outside USA)
Fax: 619-582-0919 Email to us
CONTROLLED FLOW PERFUSION SYSTEM
This is a perfusion system for precise linear control of solution flow rate over the range from 0.34 µl/min to 22 ml/min. Precision design and miniature size minimize pulsations to provide smooth perfusion. Designed for stable solution flow or substance application during imaging, calcium and other ions measurement, recording, biochemical assays or small organs and animals perfusion. Used with small chambers in lab-on-chip setups, imaging and recording workstations, and to perfuse small organs and animals in micro-perfusion setups. Ten-turn dials (or analog inputs) allow to calibrate each channel independently and to apply one or multiple substances by switching channels manually or through data acquisition and imaging software. Manual single units can be also used as a source of vacuum to remove solution from your sample chambers during perfusion because the units are so small that they can be mounted next to your samples.. You can accurately mix different solutions or generate dose-response curves using only two solutions: control and concentrated test compound. You can even generate concentration gradients in time to simulate substance distribution in time, and concentration gradients throughout the space of small chambers to assess chemotaxis, for example. Each channel can be controlled manually, by analog signal, TTL or through RS232 connection for fully automated setups controlled through our or third party software packages, imaging for example (optional USB adapters are also available). Multipple units can be attached to each other to form a multi-channel system. The size of the 2-channel unit is 4x2.5x1.85 in (separate from the controller). The controller can read analog signals (temperature, for example) to automate environment control. The controllers can be also used with our optional disposable flow meters and high power switch to setup an automated system to measure and dispense solutions using high flow rate pumps in the range from 70ml/min to 25 l/min, with precision of 200µl.
MICROMANIPULATOR for PATCH/SLICE-CLAMP, no drift manipulator
This is an extremely stable micromanipulator. MLW-3 uses water instead of oil in the hydraulic system.
The expansion coefficient of water is 1/4 that of oil. As a result, the drift is reduced by four times. The size of the drive unit
cartridge is five times greater than the size of operating cartridge. This reduces drift additional five times. The principle of leverage
is adopted in the drive unit, which reduces drift by another five times - total of hundred times reduction in drift. Movement range:
coarse - 30 mm (X, Y, and Z), fine - 400 µm (X, Y, Z). Full rotation of knob (fine) - 10 µm, (coarse) - 4 mm. Minimum
graduation - 40 nm. Dimensions: control unit - W110 x D110 x H132 mm, drive unit - W170 x D175 x H100 mm. Weight: control unit -
1.60 kg, drive unit - 1.15 kg
LOW PRICED STEREO and INVERTED MICROSCOPES
We offer high quality stereo and inverted microscopes at moderate prices.
Stereo microscopes are equipped with five different magnification objectives (switch-able) and come with a set of five eyepieces (8X and 15X).
The magnification ranges from 3.3X to 100.8X for sf-100 models
(working distance - 95 mm, prices start from $660.25), and
from 2.4X to 47.2X for sf-50 models (working distance - 170 mm, prices start from $565.25).
The fully equipped inverted microscope costs
only $3,277.50 and comes with four objectives (working distance from 20 to 3
mm), two attachments for phase contrast and polarization, illuminator, a set of
six filters and an additional eyepiece with two interchangeable measuring
reticles (item# in-iq902).
MICROMANIPULATOR with ANTI-VIBRATION DESIGN
Nmn-25 is a mechanical micromanipulator suitable for both conventional patch
clamping or slice patch. Compact model with the high performance of the NMN-21 manipulator.
Anti-vibration design was used in the body construction. Many manipulators can
be mounted on the microscope stage. The mechanical formula provides stable
performance and does not generate electrical noise. Detachable electrode holder
fixing part could be rotated for easy electrode exchange. Working distance: X/Z axis
(Coarse) 15mm X/Z axis (Fine) 6mm; one rotation of fine control knob - 250µm.
Y-axis (Coarse and Fine) - 10mm; one rotation of fine control knob - 292µm.
PERFUSION CHAMBER-INSERT for PETRI DISH - PCP1
Cells cultured in 35 mm Petri dishes are a popular research tool used in
numerous applications, including patch clamping and intracellular ion probe
imaging. However, true perfusion (continuous inflow and outflow) of solutions
can be difficult to configure. Drug delivery without an outflow requires
a Picospritzer-type microinjector, but ultimately results in contamination
of the entire dish after only a few applications. This forces scientists
to plate cells on coverslips for placement into specially designed
perfusion chambers. However, such transfer is a time consuming process
which introduces the potential for contamination plus additional expense.
The PCP1 chamber was designed by scientists after several years of
patch clamp research combined with external perfusion of single cells
cultured in Petri dishes. The chamber has separate openings for solution
inflow and outflow that dampen the fluctuations of the liquid level in the
working compartment and prevents bubbles from entering the chamber. An
adjustable metal suction tube (included) helps to control the level of liquid in the dish. The chamber was designed for use
with
automated perfusion systems
and can accommodate commercially available Teflon manifolds (optional).
Choose from chambers to fit Corning, Falcon, or Nunc 35 mm dishes. The
chamber also fits to thin coverslip bottom dishes and
could be a perfect companion for those who want to change solutions during fluorescence
experiments.
Bath Chamber with Glass coverslip bottom - PCCS1
The most flexible and easy to use perfusion chamber-assembly for upright and inverted microscopes. To accommodate the wide range
of possible applications, this system consists of three movable and replaceable elements: perfusion chamber, magnetic adjustable
tubing/electrode holders and a stainless microscope stage insert. It could be used with upright or inverted microscopes,
fluorescent or water immersion objectives, tissue slices or single cells, and could be used as stand alone or could be easily
adapted to perfusion systems.
OOCYTE PERFUSION CHAMBER - OPC3
Expression of recombinant receptors in oocytes have
been one of the favorite choices for researchers to assess the pharmacology of recombinant receptors.
Since the oocytes are 1 - 1.5 mm in diameter, however, the fast solution exchange around the oocyte
could be a problem. After several years of electrophysiological research,
we have designed a Xenopus oocyte perfusion chamber for use in
automated and unattended experiments. One of the major characteristic of the
chamber presented is a small working volume (~20 μl), which allows the fast solution exchanges.
Combined with automated perfusion systems, this chamber lets the researcher to obtained dose-response
data fast and easily. It could be also used with Voltage Clamp amplifiers, which
employ two electrodes bath clamp.
PATCH CLAMP AMPLIFIER WPC-100
WPC-100 is an excellent patch clamp amplifier for single channel and whole cell patch clamping
in both voltage clamp and current clamp modes. The amplifier noise in the patch mode is
only 15 fA rms at 1 kHz bandwidth (4-pole Bessel filter, open headstage).
Works with any data acquisition software and hardware.
Small Volume Perfusion System - SVDS1
The majority of available perfusion systems have long lines of tubing, which have to be filled before conducting experiments.
However, sometimes applied substances are available only in limited quantities or are extremely expensive, prohibiting the usage
of conventional perfusion systems. To minimize the waste of applied substances, we have designed the 8-channel small volume
delivery system. The system utilizes small plastic tubes with conical bottom and thin polyimide tubing to connect to a maltibarrel
micropipette or a micromanifold. It could be used with as little as 100
µl volumes of perfusate.
AUTOMATED GEL/BLOT WASHING
ValveLink or
ValveBank perfusion systems can automate
washing of electrophoresis gels and blots, and developing of x-ray or photographic film or prints.
You have full control over washing steps of your Western, Northern, Southern, Coomassie
Blue and Silver staining. You decide which runs are automated and which are handled
conventionally. Unattended washing can save countless hours of tedious
monitoring for a busy lab.
We currently offer inexpensive Imaging System for both bright light and fluorescent microscopy. The system is
based on high resolution Sony CCD camera and imaging software developed in NIH. It is perfect for image documentation, FISH, images
superimposition and even 3-D reconstruction. The cost for the system is only $4,800, for systems shipped installed in the
computer - $7,395, and for the system including the computer and the fluorescent microscope - $14,200. See an
example of
fluorescent image obtained using our microscope. The image is the result of superimposition of two
images obtained with included filter sets for FITC and DAPI. The subject is BPAE cells stained with FL phallacidin for
F-actin and DAPI for nuclei (Molecular Probes).
Inexpensive epi-fluorescence microscope
comes complete with a set of filter cubes for common probes, eight fluorescence objectives from x10 to x100 and phase
contrast ($6,890). Trinocular microscopes could be configured with complete imaging systems (from $4,800.) Filters for other
probes are available, including multi-band filter sets. Could be used for FISH and other applications.
NEW IMAGING SYSTEMS
Choose from basic and inexpensive to high performance and high resolution
complete imaging systems and accessories.
We offer systems for image documentation and measurement, ratiometric fluorescence, 3-D reconstruction and mosaic imaging of wide
samples. Accessories range from filter weels and motorized stages to deconvolution software and include bath
chambers, perfusion
systems and temperature control, including heaters for objective lenses.
Complete systems are based on either
Olympus IX71 inverted or BX51WI upright microscopes. Basic systems include our inexpensive fluorescent or inverted microscopes.
An ergonomic inverted microscope with motorized control of the stage and focus
movement, which eliminate the need to elevate and to pool your arms to reach the
microscope handles. Allows to position the microscope into remote areas for control from the
distance - sterile rooms, for example.
CONTROLLED ENVIRONMENT CLOSED IMAGING CHAMBER
Could be
used with four standard 25 mm glass coverslips to form optical quality surfaces
on the bottom and on the top of the media and above the gas mixture to control
the atmosphere (pH) and to prevent condensation. Easy to assemble. Includes
stainless suction tubing for continuous media exchange and luer connector for
gas mixture input (from a cylinder with CO2/O2, for
example). Easily converts to sealed or open chamber. The chamber has separate
compartments for solution inflow and outflow to provide smooth perfusion and to
prevent bubbles from coming into the working area. Consider adding a miniature perfusion
system. Fits into heating stages of our temperature controllers.
CONTROLLED FLOW PERFUSION SYSTEM
This is a perfusion system for precise linear control of solution flow rate over the range from 0.34 µl/min to 22 ml/min. Precision design and miniature size minimize pulsations to provide smooth perfusion. Designed for stable solution flow or substance application during imaging, recording, calcium and other ions measurement, biochemical assays or small organs and animals perfusion. Used with small chambers in lab-on-chip setups, imaging and recording workstations, and to perfuse small organs and animals in micro-perfusion setups. Ten-turn dials (or analog inputs) allow to calibrate each channel independently and to apply one or multiple substances by switching channels manually or through data acquisition and imaging software. You can accurately mix different solutions or generate dose-response curves using only two solutions: control and concentrated test compound. You can even generate concentration gradients in time to simulate substance distribution in time, and concentration gradients throughout the space of small chambers to assess chemotaxis, for example. Each channel can be controlled manually, by analog signal, TTL or through RS232 connection for fully automated setups controlled through third party software packages, imaging for example (optional USB adapters are also available). Multiple systems could be attached to each other to form a multi-channel system. The size of the 2-channel system is 4x2.5x1.85 in (separate from the controller). Can read up to eight analog signals (temperature, for example) to automate environment control. Can be used with our optional disposable flow meters and high power switch to setup an automated system to measure and dispense solutions using high flow rate pumps in the range from 70ml/min to 25 l/min, with precision of 200µl.
COMPLETE PATCH CLAMP WORKSTATION for $18,240
This station includes inverted phase contrast microscope with four long-distance objectives (x20 max), no-drift micromanipulator, patch clamp amplifier, data acquisition interface and software. The manipulator mounts directly on the microscope stage for stable no-drift and no-vibration operations. Shipped installed on the latest model of PC computer with CD-writer and three 1200GB of hard drives space to accommodate data storage needs. The system can be modified to include other components. Works with our perfusion systems and temperature controlled chambers.
CONTROLLED FLOW PERFUSION SYSTEM
This is a perfusion system for precise linear control of solution flow rate
over the range from 0.34 µl/min to 22 ml/min. Precision design and miniature size minimize pulsations to provide smooth perfusion.
Designed for stable solution flow or substance application during
imaging, recording, calcium and other ions measurement, biochemical
assays or small organs and animals perfusion.
Used with small chambers in lab-on-chip setups, imaging and recording workstations, and to perfuse small organs and animals in
micro-perfusion setups. Ten-turn dials (or analog inputs) allow to calibrate each channel independently
and to apply one or multiple substances by switching channels manually
or through data acquisition and imaging software.
You can accurately mix different solutions or generate dose-response curves using only two solutions: control and
concentrated test compound. You can even generate concentration gradients in time to simulate substance distribution in time,
and concentration gradients throughout the space of small chambers to assess chemotaxis, for example.
Each channel can be controlled manually, by analog signal, TTL or through RS232 connection for fully automated setups controlled through third party software packages, imaging for example (optional USB adapters are also available). Multiple systems could be attached to each other to form a multi-channel system. The size of the 2-channel system is 4x2.5x1.85 in (separate from the controller). Can read up to eight analog signals (temperature, for example) to automate environment control. Can be used with our optional disposable flow meters and high power switch to setup an automated system to measure and dispense solutions using high flow rate pumps in the range from 70ml/min to 25 l/min, with precision of 200µl.
Automated Workstations
Automation Software
Perfusion Systems
Flow Control
Temperature Control
Miniature Holders
Miniature Manifolds
Local Perfusion
Small Volume Delivery System
Perfusion Chambers for replaceable coverslip
Ultra-Thin Imaging Chambers
Closed Controlled Environment Chambers & Mini-Incubators
Gradient Chambers
Uniform Heating
Slide Heaters
Micro-Perfusion
Automated Workstations
Multi-Channel Systems
Chambers, Thin profile, Heated, Perfusion, for AFM and Water Immersion
Accessories
Low Profile Heated Chambers
Data Acquisition
Computer-based Data Acquisition
Electrophysiology Accessories
Accessories
Electrophysiology Workstations
Patch Clamp
Experimental Rigs & Faraday Cages
Faraday Cages
Flash Photolysis System
The Xenon Arc Flash Photolysis System
Fluorescence Workstations
Monochromator Based Systems
Glass Bottom Petri Dishes
Accessories
Sterile Dishes
Gradient Chambers
Accessories
Imaging Chambers
Imaging Slides, Heating & Accessories
Temperature Control & Holders
Imaging Systems
Color Imaging Systems
Enhanced Imaging Systems
Imaging, High Resolution Chambers
Accessories
Chambers with Transparent Uniform Heaters
Heated Chambers
Open Chambers
Thin Sealed Chambers
Injection and Micromanipulation Workstations
Accessories
Inverted Phase Contrast and DIC microscope stations
Stereo Microscope Stations
Injectors
Accessories
Complete Injection Systems
Microinjectors
Magnetic & Screw-Type Holders, Miniature
Accessories
Holders
Manipulators, Accessories
Accessories for UM series
Headstage Adapters and Electrode Holders
Magnetic Stands, Clamps and Joints
Manipulators, Coarse
One-Dimensional
Three-Dimensional
Two-Dimensional
Manipulators, Fine Mechanical
Joystic Type
One-Dimensional
Three-Dimensional
Two-Dimensional
Manipulators, High Resolution Motorized
Micromanipulators
Manipulators, Hydraulic
Single-Axis
Three-axis
Manipulators, Microscope Adapters
Isolation Systems
Leica Microscopes
Nikon Microscopes
Olympus Microscopes
Zeiss Microscopes
Manipulators, Miniature Compact
Accessories
Installation
One-Dimensional
Three-Dimensional
Manipulators, Motor-driven and Piezo-Injectors
Motor Drive
Piezoelectric
Manipulators, Multiple Pipettes
Holders
Manipulators
Membrane capacitance measurements
Optopatch Amplifier
Micropipette Pullers, Microforges and Grinders
Capillaries for Injection
Grinders
Microforges
Multibarrel Capillaries
Patch Clamp Glass Capillaries
Pullers
Microscope Accessories
Eyepieces
Heads
Miscellaneous
Photo/Video Adapters
Reticles
Upgrade Packages
Microscope/Manipulators Isolation/Translation Tables
Isolation Table
Microscope Translation Stages
X-Y Base
Microscopes, Fluorescence
Filters and Objectives
Fluorescence Microscopes
Hybridization and Incubation on Slides
Live Imaging Chambers
Microscopes, Inverted
Biological Inverted Microscopes
Inverted Material Research Microscopes
Microscopes, Laboratory
Fluorescence Microscopes
Laboratory Microscopes
Material Research Microscopes
Objectives
Microscopes, Stereo
Accessories
Stereo Microscopes
Optical Monochromators
Monochromator Systems
Patch Clamp Amplifiers
Data Acquisition Interfaces
Patch Clamp Amplifier
Perfusion Accessories, Micro-Manifolds and Tubing
micro-Manifolds
Miscellaneous
Perfusion Pumps
Small Volume Delivery Systems
Tubing & Fitting
Perfusion Chambers
Controlled Environment Closed Imaging Chambers
CoverSlip Chambers
Oocyte chambers
Petri Dish Inserts
Perfusion Dish for High Resolution Imaging
P-Dish
Perfusion Systems, Fast and Miniature
1.5 msec response time
10-25 msec response time
Accessories
Pinch Valves Perfusion Systems
Perfusion Systems, Flow Control
Accessories
Miniature Perfusion Systems
Perfusion systems, Intracellular
Pipette Adapter Based
Perfusion Systems, Micro Perfusion Sets
Accessories
Micro Perfusion Sets
Perfusion Systems, Ultra-fast
Sub-Milisecond Solution Switch System
Pressure Micro Injectors - Spritzers
1-Channel Injectors
4, 8-channel Micro Injectors - Spritzers
Accessories
Software
Automation Software
Data Acquisition
Imaging Software
Stimulators and Isolators
Microforge for Metal Electrodes
Stimulators and Isolators
Stimulus Electrodes
Temperature Controllers, Heating
1-Channel Controller, Low Electrical Noise
2-Channel Temperature Controller
Accessories
Miniature Temperature Controller
Ultra Fast Temperature Change
Temperature Controllers, Heating & Cooling
Bipolar Heating/Cooling Controllers
Two Electrode Voltage Clamp/Oocyte Amplifier
Voltage Clamp Amplifier
Vibration Isolation Tables
Accessories
Air Vibration Isolation Lab Tables
CB Consulting Inc.
